King's Highway 53, commonly referred to as Highway 53, was a provincially maintained highway in the southern portion of the Canadian province of Ontario that connected Woodstock to Hamilton via Brantford. The 76.5-kilometre (47.5 mi) route served as a southerly bypass to Highway 2, avoiding Paris, Ancaster and Hamilton. In addition to the previously mentioned cities, Highway 53 served the communities of Cathcart and Burford.