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Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension.

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  • Nanopartikel-Tracking-Analyse (de)
  • Nanoparticle tracking analysis (it)
  • Nanoparticle tracking analysis (en)
  • Анализ траекторий наночастиц (ru)
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  • Die Nanopartikel-Tracking-Analyse (englisch Nanoparticle tracking analysis) (NTA) beschreibt eine analytische Methode zur Bestimmung von Partikelgrößen und flüssigen Medien. Hierfür wird die Brownsche Molekularbewegung genutzt, da deren Größe bei konstanter Temperatur allein von der Partikelgröße abhängig ist. Durch eine optische Bildgebung werden die Bewegungsgeschwindigkeiten der einzelnen Partikel verfolgt, also getrackt, und durch den Computer ausgewertet. Dadurch kann eine Partikelgrößenverteilung gewonnen werden. (de)
  • Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension. (en)
  • La Nanoparticle Tracking Analysis (NTA), in italiano traducibile con "analisi per il monitoraggio delle nanoparticelle", è una tecnica sviluppata dalla NanoSight Ltd che può essere utilizzata per determinare il profilo della distribuzione delle dimensioni di piccole particelle sospese in un liquido. (it)
  • Анализ траекторий наночастиц — метод визуализации и изучения наночастиц в растворах, разработанный компанией Nanosight (Великобритания). В его основе лежит наблюдение за Броуновским движением отдельных наночастиц, скорость которого зависит от вязкости и температуры жидкости, а также размера и формы наночастицы. Это позволяет использовать данный принцип для измерения размера наночастиц в коллоидных растворах. В дополнение к размеру, одновременно возможно измерение интенсивности рассеяния света индивидуальной наночастицей, что позволяет дискриминировать наночастицы по их материалу. Третьим измеряемым параметром является концентрация каждой из фракций наночастиц. (ru)
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  • Die Nanopartikel-Tracking-Analyse (englisch Nanoparticle tracking analysis) (NTA) beschreibt eine analytische Methode zur Bestimmung von Partikelgrößen und flüssigen Medien. Hierfür wird die Brownsche Molekularbewegung genutzt, da deren Größe bei konstanter Temperatur allein von der Partikelgröße abhängig ist. Durch eine optische Bildgebung werden die Bewegungsgeschwindigkeiten der einzelnen Partikel verfolgt, also getrackt, und durch den Computer ausgewertet. Dadurch kann eine Partikelgrößenverteilung gewonnen werden. Mittlerweile existiert eine verbesserte Methode der NTA, bekannt unter der Bezeichnung „interferometrisches NTA“ (iNTA). Hierbei nutzt man das Prinzip der , um das schwache Streulicht des Partikels zu verstärken. Im Gegensatz zu NTA, bietet iNTA eine wesentlich besseres Auflösungsvermögen, was zum einen durch die höhere Bildrate (5000 Bilder pro Sekunde; NTA: 25–60 Bilder pro Sekunde), aber auch durch den Partikelkontrast realisiert wird. (de)
  • Nanoparticle tracking analysis (NTA) is a method for visualizing and analyzing particles in liquids that relates the rate of Brownian motion to particle size. The rate of movement is related only to the viscosity and temperature of the liquid; it is not influenced by particle density or refractive index. NTA allows the determination of a size distribution profile of small particles with a diameter of approximately 10-1000 nanometers (nm) in liquid suspension. The technique is used in conjunction with an ultramicroscope and a laser illumination unit that together allow small particles in liquid suspension to be visualized moving under Brownian motion. The light scattered by the particles is captured using a CCD or EMCCD camera over multiple frames. Computer software is then used to track the motion of each particle from frame to frame. The rate of particle movement is related to a sphere equivalent hydrodynamic radius as calculated through the Stokes–Einstein equation. The technique calculates particle size on a particle-by particle basis, overcoming inherent weaknesses in ensemble techniques such as dynamic light scattering. Since video clips form the basis of the analysis, accurate characterization of real time events such as aggregation and dissolution is possible. Samples require minimal preparation, minimizing the time required to process each sample. Speculators suggest that eventually the analysis may be done in real-time with no preparation, e.g. when detecting the presence of airborne viruses or biological weapons. NTA currently operates for particles from about 10 to 1000 nm in diameter, depending on particle type. Analysis of particles at the lowest end of this range is possible only for particles composed of materials with a high refractive index, such gold and silver. The upper size limit is restricted by the limited Brownian motion of large particles; because a large particle moves very slowly, accuracy is diminished. The viscosity of the solvent also influences the movement of particles, and it, too, plays a part in determining the upper size limit for a specific system. (en)
  • La Nanoparticle Tracking Analysis (NTA), in italiano traducibile con "analisi per il monitoraggio delle nanoparticelle", è una tecnica sviluppata dalla NanoSight Ltd che può essere utilizzata per determinare il profilo della distribuzione delle dimensioni di piccole particelle sospese in un liquido. La tecnica viene utilizzata in combinazione con un che permette di visualizzare il movimento delle piccole particelle in sospensione liquida sotto l'effetto del moto browniano. Il software del computer viene quindi ad essere utilizzato per tenere traccia dei movimenti delle particelle e successivamente valutarne il loro raggio idro-dinamico utilizzando l'equazione di . Inoltre, dato che i campioni richiedono una preparazione minima, il tempo richiesto per elaborarli è molto ridotto. Gli osservatori suggeriscono che alla fine l'analisi può essere effettuata in tempo reale senza alcuna preparazione, per es. quando si viene a rilevare la presenza di virus o di armi biologiche nell'aria. (it)
  • Анализ траекторий наночастиц — метод визуализации и изучения наночастиц в растворах, разработанный компанией Nanosight (Великобритания). В его основе лежит наблюдение за Броуновским движением отдельных наночастиц, скорость которого зависит от вязкости и температуры жидкости, а также размера и формы наночастицы. Это позволяет использовать данный принцип для измерения размера наночастиц в коллоидных растворах. В дополнение к размеру, одновременно возможно измерение интенсивности рассеяния света индивидуальной наночастицей, что позволяет дискриминировать наночастицы по их материалу. Третьим измеряемым параметром является концентрация каждой из фракций наночастиц. Метод активно набирает популярность в научной среде. Так, на начало осени 2012 года количество научных публикаций с использованием метода Анализа траекторий наночастиц достигло 400, из них более 100 — только за 2012 год. (ru)
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