Route 64 is a highway in central Missouri with endpoints of Route 254 south of Hermitage and Route 5 in Lebanon. Route 64 is one of the original 1922 state highways and originally ran between Preston and Collins. It would eventually be moved further to the south with its older alignment becoming U.S. Route 54. It would also be extended east. It is also only one of three Missouri state highways bearing the same number as an Interstate highway in Missouri.