Route 164 is a state highway in the Missouri Bootheel. The route starts at U.S. Route 412 (US 412) in Cardwell. The route travels eastward across the bootheel, and it goes through the towns of Arbyrd, Hornersville, Rives, and Steele. It becomes concurrent with US 61 briefly in Steele, and intersects Interstate 55 (I-55) east of the city. The route ends east of Cottonwood Point, near the Mississippi River.