Little Buffalo Creek is a tributary of Buffalo Creek in Union County, Pennsylvania, in the United States. It is approximately 10 miles (16 km) long and flows through White Deer Township and Kelly Township. The watershed of the creek has an area of 19.0 square miles (49 km2). The creek is impacted by nutrients, sediment, E. coli, and thermal radiation. Rock formations containing sandstone, shale, and limestone occur in the watershed.