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The Isolation chip (or ichip) is a method of culturing bacteria. Using regular methods, 99% of bacterial species are not able to be cultured as they do not grow in conditions made in a laboratory, a problem called the "Great Plate Count Anomaly". The ichip instead cultures bacterial species within its soil environment. The soil is diluted in molten agar and nutrients such that only a single cell, on average, grows in the ichip's small compartments or wells, hence the term "isolation". The chip is then enclosed in a semipermeable plastic membrane and buried back in the dirt to allow in nutrients not available in the lab. With this culturing method, about 50 to 60 percent of bacterial species are able to survive. Notably, the bacterial species Eleftheria terrae, which makes the antibiotic te

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  • Isolation chip (en)
  • Isolation chip (fr)
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  • The Isolation chip (or ichip) is a method of culturing bacteria. Using regular methods, 99% of bacterial species are not able to be cultured as they do not grow in conditions made in a laboratory, a problem called the "Great Plate Count Anomaly". The ichip instead cultures bacterial species within its soil environment. The soil is diluted in molten agar and nutrients such that only a single cell, on average, grows in the ichip's small compartments or wells, hence the term "isolation". The chip is then enclosed in a semipermeable plastic membrane and buried back in the dirt to allow in nutrients not available in the lab. With this culturing method, about 50 to 60 percent of bacterial species are able to survive. Notably, the bacterial species Eleftheria terrae, which makes the antibiotic te (en)
  • L'isolation chip ou ichip est un dispositif pour l'obtention de cultures bactériennes en milieu naturel. Son emploi se révèle approprié du fait qu'une faible fraction des populations de bactéries peuvent être cultivées dans un milieu artificiel. Le dispositif permet aux espèces bactériennes d'être cultivées dans leur environnement pédologique. Les échantillons microbiens prélevés sur le terrain sont transférés sur un gel d'agarose et enveloppés de membranes semi-perméables. Cette nouvelle approche résulte de l'application dans ce domaine de la microfluidique. Mise au point en 2002 par Kim Lewis et Slava Epstein, l'une des raisons de son utilisation se fonde sur l'espoir de la découverte de nouveaux antibiotiques voire de nouvelles classes d'antibiotiques. Eleftheria terrae est l'une des es (fr)
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  • The Isolation chip (or ichip) is a method of culturing bacteria. Using regular methods, 99% of bacterial species are not able to be cultured as they do not grow in conditions made in a laboratory, a problem called the "Great Plate Count Anomaly". The ichip instead cultures bacterial species within its soil environment. The soil is diluted in molten agar and nutrients such that only a single cell, on average, grows in the ichip's small compartments or wells, hence the term "isolation". The chip is then enclosed in a semipermeable plastic membrane and buried back in the dirt to allow in nutrients not available in the lab. With this culturing method, about 50 to 60 percent of bacterial species are able to survive. Notably, the bacterial species Eleftheria terrae, which makes the antibiotic teixobactin that has shown promise against many drug-resistant strains like methicillin-resistant Staphylococcus aureus, was discovered using the ichip in 2015. In addition to antibiotics, it is argued that anti-cancer agents, anti-inflammatory and immunosuppressives (which have previously been discovered from bacteria) as well as potential energy sources could be discovered. The ichip was developed by the drug discovery company NovoBiotic Pharmaceuticals, founded by Kim Lewis and Slava Epstein. (en)
  • L'isolation chip ou ichip est un dispositif pour l'obtention de cultures bactériennes en milieu naturel. Son emploi se révèle approprié du fait qu'une faible fraction des populations de bactéries peuvent être cultivées dans un milieu artificiel. Le dispositif permet aux espèces bactériennes d'être cultivées dans leur environnement pédologique. Les échantillons microbiens prélevés sur le terrain sont transférés sur un gel d'agarose et enveloppés de membranes semi-perméables. Cette nouvelle approche résulte de l'application dans ce domaine de la microfluidique. Mise au point en 2002 par Kim Lewis et Slava Epstein, l'une des raisons de son utilisation se fonde sur l'espoir de la découverte de nouveaux antibiotiques voire de nouvelles classes d'antibiotiques. Eleftheria terrae est l'une des espèces de bactérie à avoir été cultivée grâce à ce procédé et, de cette culture bactérienne, les chercheurs ont découvert la teixobactine, un antibiotique capable d'éliminer les bactéries responsables de l'anthrax et de la tuberculose de même que celles à l'origine des infections à SARM (staphylococcus aureus résistant à la méticilline). (fr)
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