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Gmelin's test is a chemical test used for detecting the presence of bile pigments in urine. It is named after Leopold Gmelin, who introduced the test. Five millilitres of urine is slowly added to five millilitres of concentrated nitric acid in a test-tube. Different coloured rings between the two layers are visible if bile pigments are present as they are oxidised to various chemical products. Nitric acid is used as the oxidising agent. Blue, green and violet rings are seen if bilirubin is present. Gmelin's test is not sensitive so a positive result always indicates the presence of bile pigments but a negative result does not exclude the presence of small quantities of bile pigments.

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  • Prueba de Gmelin (es)
  • Gmelin's test (en)
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  • Gmelin's test is a chemical test used for detecting the presence of bile pigments in urine. It is named after Leopold Gmelin, who introduced the test. Five millilitres of urine is slowly added to five millilitres of concentrated nitric acid in a test-tube. Different coloured rings between the two layers are visible if bile pigments are present as they are oxidised to various chemical products. Nitric acid is used as the oxidising agent. Blue, green and violet rings are seen if bilirubin is present. Gmelin's test is not sensitive so a positive result always indicates the presence of bile pigments but a negative result does not exclude the presence of small quantities of bile pigments. (en)
  • La prueba de Gmelin es una prueba química que se utiliza para detectar la presencia de en la orina. Lleva el nombre de Leopold Gmelin, quien introdujo la prueba.​​​ Se agregan lentamente cinco mililitros de orina a cinco mililitros de ácido nítrico concentrado en un tubo de ensayo. Los anillos de diferentes colores entre las dos capas son visibles si hay pigmentos biliares presentes, ya que se oxidan a varios productos químicos.​​ El ácido nítrico se utiliza como agente oxidante.​ Se ven anillos azules, verdes y violetas si hay bilirrubina.​ La prueba de Gmelin no es sensible, por lo que un resultado positivo siempre indica la presencia de pigmentos biliares, pero un resultado negativo no excluye la presencia de pequeñas cantidades de pigmentos biliares.​ (es)
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  • Gmelin's test is a chemical test used for detecting the presence of bile pigments in urine. It is named after Leopold Gmelin, who introduced the test. Five millilitres of urine is slowly added to five millilitres of concentrated nitric acid in a test-tube. Different coloured rings between the two layers are visible if bile pigments are present as they are oxidised to various chemical products. Nitric acid is used as the oxidising agent. Blue, green and violet rings are seen if bilirubin is present. Gmelin's test is not sensitive so a positive result always indicates the presence of bile pigments but a negative result does not exclude the presence of small quantities of bile pigments. (en)
  • La prueba de Gmelin es una prueba química que se utiliza para detectar la presencia de en la orina. Lleva el nombre de Leopold Gmelin, quien introdujo la prueba.​​​ Se agregan lentamente cinco mililitros de orina a cinco mililitros de ácido nítrico concentrado en un tubo de ensayo. Los anillos de diferentes colores entre las dos capas son visibles si hay pigmentos biliares presentes, ya que se oxidan a varios productos químicos.​​ El ácido nítrico se utiliza como agente oxidante.​ Se ven anillos azules, verdes y violetas si hay bilirrubina.​ La prueba de Gmelin no es sensible, por lo que un resultado positivo siempre indica la presencia de pigmentos biliares, pero un resultado negativo no excluye la presencia de pequeñas cantidades de pigmentos biliares.​ (es)
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