The 2016 Southland Conference tournament was held at Cowgirl Diamond on the campus of McNeese State University in Lake Charles, Louisiana from May 11 through May 13, 2016. The tournament winner, the McNeese State Cowgirls, earned the Southland Conference's automatic bid to the 2016 NCAA Division I softball tournament. The Championship game was broadcast on ESPN3 with the remainder of the tournament airing on the Southland Digital Network.