The 58th Mobil 1 12 Hours of Sebring presented by Fresh from Florida was the 2010 running of the 12 Hours of Sebring and the opening round of the 2010 American Le Mans Series season. It took place at the Sebring International Raceway, Sebring, Florida on March 20, 2010. The race featured the debut of the Le Mans Prototype Challenge category for spec racing.