The 2009 NCAA Division I Women's Lacrosse Championship was the 28th annual single-elimination tournament to determine the national champion of Division I NCAA women's college lacrosse. The tournament was played from May 10 to May 24, 2009, and the semifinal and championship rounds were played at Johnny Unitas Stadium in Towson, Maryland from May 22–24. All NCAA Division I women's lacrosse programs were eligible for this championship, and a total of 16 teams were invited to participate.