The 2009–10 Duquesne Dukes men's basketball team represented Duquesne University in 2009–10 NCAA Division I men's basketball season. Duquesne hoped to build on the success of the 2008–09 season and began the new year with five straight victories. Soon, however, a string of setbacks hit the team, including the loss of two players, one due to injury and the other to suspension. Duquesne lost its final non-conference game and slipped into a five-game losing streak. The team did manage to break the streak, but had trouble gaining any momentum. In mid-February the Dukes finally seemed to break free with a solid 21 point victory over La Salle, followed by a crucial road upset of the University of Charlotte, which was ranked first in the Atlantic 10 Conference at the time, and finally another ups