The 2000 UCLA Bruins softball team represented the University of California, Los Angeles in the 2000 NCAA Division I softball season. The Bruins were coached by Sue Enquist, in her twelfth season as head coach. The Bruins played their home games at Easton Stadium and finished with a record of 46–12–1. They competed in the Pacific-10 Conference, where they finished third with a 14–7 record.