The 2000 Pepsi Southern 500 presented by Kmart, the 51st running of the event, was the 24th race of the 2000 NASCAR Winston Cup Series season. It was held at Darlington Raceway on September 3, 2000. The race was scheduled for 367 laps but was shortened to 328 laps due to rain. Bobby Labonte of Joe Gibbs Racing won the race.