The 2000 Football League Second Division play-off Final was an association football match played at Wembley Stadium on 28 May 2000, to determine the third and final team to gain promotion from the Second Division to the First Division of the Football League in the 1999–2000 season. Gillingham faced Wigan Athletic in one of the last competitive games to be played at the original Wembley Stadium. It was Gillingham's second consecutive appearance in the Second Division play-off final after a defeat to Manchester City a year earlier. Wigan had been defeated in the semi-finals the previous season and the game marked their first appearance in a play-off final. The teams reached the 2000 final by defeating Stoke City and Millwall respectively in the semi-finals.