The 2000–01 Creighton Bluejays men's basketball team represented Creighton University during the 2000–01 NCAA Division I men's basketball season. The Bluejays, led by head coach Dana Altman, played their home games at the Omaha Civic Auditorium. The Jays finished with a 24-8 record, and won the Missouri Valley Conference regular season title to earn an at-large bid to the 2001 NCAA tournament.