The 1964 Wyoming Cowboys football team represented the University of Wyoming in the Western Athletic Conference (WAC) during the 1964 NCAA University Division football season. In their third season under head coach Lloyd Eaton, the Cowboys compiled a 6–2–2 record (2–2 in WAC, fourth), and outscored opponents 181 to 117. Dick Barry, Jeff Hartman, and Bill Levine were the team captains.