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About: Relative fluorescence units     Goto   Sponge   NotDistinct   Permalink

An Entity of Type : yago:Technique105665146, within Data Space : dbpedia.org associated with source document(s)

Type: yago:WikicatLaboratoryTechniquesyago:Ability105616246yago:Abstraction100002137yago:Cognition100023271yago:Know-how105616786yago:Method105660268yago:PsychologicalFeature100023100yago:Technique105665146 New Facet based on Instances of this Class

The terms "relative fluorescence units" (RFU) and "RFU peak" refer to measurements in electrophoresis methods, such as for DNA analysis. A "relative fluorescence unit" is a unit of measurement used in analysis which employs fluorescence detection. Fluorescence is detected using a charged coupled device (CCD) array, when the labeled fragments, which are separated within a capillary by using electrophoresis, are energized by laser light and travel across the detection window. A computer program measures the results, determining the quantity or size of the fragments, at each data point, from the level of fluorescence intensity. Samples which contain higher quantities of amplified DNA will have higher corresponding RFU values.