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The Lamm equation describes the sedimentation and diffusion of a solute under ultracentrifugation in traditional sector-shaped cells. (Cells ofother shapes require much more complex equations.) It was named after Ole Lamm, later professor of physical chemistry at the Royal Institute of Technology, who derived it during his Ph.D. studies under Svedberg at Uppsala University. The Lamm equation can be written: where kBT is the thermal energy, i.e.,Boltzmann's constant kB multiplied bythe temperature T in kelvins.

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  • Lamm equation (en)
  • Équation de Lamm (fr)
  • Equação de Lamm (pt)
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  • L'équation de Lamm décrit la sédimentation de particules en solution dans un liquide tel qu'elle se produit dans une centrifugeuse. Cette équation a été ainsi nommée en l'honneur du physico-chimiste suédois Ole Lamm. Elle constitue un cas particulier de l'équation de Mason-Weaver. (fr)
  • The Lamm equation describes the sedimentation and diffusion of a solute under ultracentrifugation in traditional sector-shaped cells. (Cells ofother shapes require much more complex equations.) It was named after Ole Lamm, later professor of physical chemistry at the Royal Institute of Technology, who derived it during his Ph.D. studies under Svedberg at Uppsala University. The Lamm equation can be written: where kBT is the thermal energy, i.e.,Boltzmann's constant kB multiplied bythe temperature T in kelvins. (en)
  • A equação de Lamm descreve a sedimentação e difusão de um soluto sobre em células do tipo setor tradicional. (Células de outras formas requerem equações mais complexas) A equação de Lamm pode ser escita: onde c é a concentração do soluto, t e r são o tempo e o raio e os parâmetros D, s, e representam a constante de difusão do soluto, coeficiente de sedimentação e velocidade angular do rotor, respectivamente. onde é a energia térmica, i.e., a constante de Boltzmann multiplicada pela temperatura T em kelvin. (pt)
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  • The Lamm equation describes the sedimentation and diffusion of a solute under ultracentrifugation in traditional sector-shaped cells. (Cells ofother shapes require much more complex equations.) It was named after Ole Lamm, later professor of physical chemistry at the Royal Institute of Technology, who derived it during his Ph.D. studies under Svedberg at Uppsala University. The Lamm equation can be written: where c is the solute concentration, t and r are the time and radius, and the parameters D, s, and ω represent the solute diffusion constant, sedimentation coefficient and the rotor angular velocity, respectively. The first and second terms on the right-hand side of the Lamm equation are proportional to D and sω2, respectively, and describe the competing processes of diffusion and sedimentation. Whereas sedimentation seeks to concentrate the solute near the outer radius of the cell, diffusion seeks to equalize the solute concentration throughout the cell. The diffusion constant D can be estimated from the hydrodynamic radius and shape of the solute, whereas the buoyant mass mb can be determined from the ratio of s and D where kBT is the thermal energy, i.e.,Boltzmann's constant kB multiplied bythe temperature T in kelvins. Solute molecules cannot pass through the inner and outer walls of thecell, resulting in the boundary conditions on the Lamm equation at the inner and outer radii, ra and rb, respectively. By spinning samples at constant angular velocity ω and observing the variation in the concentration c(r, t), one may estimate the parameters s and D and, thence, the (effective or equivalent) buoyant mass of the solute. (en)
  • L'équation de Lamm décrit la sédimentation de particules en solution dans un liquide tel qu'elle se produit dans une centrifugeuse. Cette équation a été ainsi nommée en l'honneur du physico-chimiste suédois Ole Lamm. Elle constitue un cas particulier de l'équation de Mason-Weaver. (fr)
  • A equação de Lamm descreve a sedimentação e difusão de um soluto sobre em células do tipo setor tradicional. (Células de outras formas requerem equações mais complexas) A equação de Lamm pode ser escita: onde c é a concentração do soluto, t e r são o tempo e o raio e os parâmetros D, s, e representam a constante de difusão do soluto, coeficiente de sedimentação e velocidade angular do rotor, respectivamente. O primeiro e sgundo tempo no lado direito da equação de Lamm são proporcionais a D e , respectivamente, e descrevem o processos de difusão e sedimentação. Considerando que sedimentação procura concentrar o soluto próximo a região externa do raio da célula, a difusão procura igualar a concentração do soluto em toda a célula. A constante de difusão D pode ser estimada a partir do e da forma do soluto, enquanto que a massa dinâmica pode ser determinada a partir do razão de s e D onde é a energia térmica, i.e., a constante de Boltzmann multiplicada pela temperatura T em kelvin. Soluto moleculares não conseguem passar através das paredes interiores e exteriores da célular, resultando em condições de contorno na equação de Lamm no raio inteno e externo, e ,respectivamente. Ao centrifugar a amostra com uma velocidade angular constante e observando a variação na concentração , pode-se estimar os parâmetros s e D e, portanto, a massa dinâmica e a forma do soluto. (pt)
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