About: LIANTI

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Linear Amplification via Transposon Insertion (LIANTI) is a linear (WGA) method. To analyze or sequence very small amount of DNA, i.e. genomic DNA from a single cell, the picograms of DNA is subject to WGA to amplify at least thousands of times into nanogram scale, before DNA analysis or sequencing can be carried out. Previous WGA methods (DOP-PCR, MDA, MALBAC) use exponential/nonlinear amplification schemes, leading to bias accumulation and error propagation. LIANTI achieved linear amplification of the whole genome for the first time, enabling more uniform and accurate amplification.

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rdfs:label	LIANTI (en)
rdfs:comment	Linear Amplification via Transposon Insertion (LIANTI) is a linear (WGA) method. To analyze or sequence very small amount of DNA, i.e. genomic DNA from a single cell, the picograms of DNA is subject to WGA to amplify at least thousands of times into nanogram scale, before DNA analysis or sequencing can be carried out. Previous WGA methods (DOP-PCR, MDA, MALBAC) use exponential/nonlinear amplification schemes, leading to bias accumulation and error propagation. LIANTI achieved linear amplification of the whole genome for the first time, enabling more uniform and accurate amplification. (en)
foaf:depiction
dcterms:subject	Genomics
Wikipage page ID	55666703 (xsd:integer)
Wikipage revision ID	986700490 (xsd:integer)
Link from a Wikipage to another Wikipage	Amplicon Qiagen MALBAC DNA dbr:Uracil In vitro Genomics Copy-number variation Nanogram Picogram Micromanipulator DMSO DNA sequencing Protease DNA analysis DNA library DNA replication origin RNA polymerase Multiple displacement amplification Single-nucleotide polymorphism Ultraviolet Sonication Reverse transcription Microfluidic device Flow sorting Double-stranded DNA RNase inhibitor Pipetting Error propagation Self annealing dbr:Whole_genome_amplification T7 promoter Tn5 transposon dbr:Cytosine_deamination
Link from a Wikipage to an external page	https://bernstein.harvard.edu/papers/2017_Chen_Science.pdf https://github.com/lh3/lianti https://www.ncbi.nlm.nih.gov/Traces/study/%3Facc=SRP102259&go=go
sameAs	LIANTI LIANTI
dbp:wikiPageUsesTemplate	dbt:Reflist
thumbnail	wiki-commons:Special:FilePath/Linear_Amplification_and_Exponential_Amplification.png?width=300
has abstract	Linear Amplification via Transposon Insertion (LIANTI) is a linear (WGA) method. To analyze or sequence very small amount of DNA, i.e. genomic DNA from a single cell, the picograms of DNA is subject to WGA to amplify at least thousands of times into nanogram scale, before DNA analysis or sequencing can be carried out. Previous WGA methods (DOP-PCR, MDA, MALBAC) use exponential/nonlinear amplification schemes, leading to bias accumulation and error propagation. LIANTI achieved linear amplification of the whole genome for the first time, enabling more uniform and accurate amplification. Shown in the figure is a simulation to illustrate the advantages of linear amplification over exponential amplification, assuming two DNA fragments A and B with replication yields of 100% and 70% per round, respectively. First, linear amplification is more even than exponential amplification. To achieve ~10,000 fold amplification of fragment A, exponential amplification results in a ratio of 8:1, hampering the accuracy of CNV detection. In contrast, linear amplification exhibits a ratio of 1:0.7, which is much closer to unity. Second, linear amplification is superior to exponential amplification in fidelity. In exponential amplification, errors generated in early cycles of amplification will be propagated permanently, leading to SNV false-positives. In contrast, in linear amplification, the errors would appear randomly at different locations in the amplicons to be easily filtered out. (en)
prov:wasDerivedFrom	wikipedia-en:LIANTI?oldid=986700490&ns=0
page length (characters) of wiki page	11276 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:LIANTI
is foaf:primaryTopic of	wikipedia-en:LIANTI

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