Immunocytochemistry (ICC) is a common laboratory technique that is used to anatomically visualize the localization of a specific protein or antigen in cells by use of a specific primary antibody that binds to it. The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated fluorophore. ICC allows researchers to evaluate whether or not cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, ICC also allows researchers to determine which sub-cellular compartments are expressing the antigen.
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| - Immunocytochimie (fr)
- Immunocytochemistry (en)
- 면역세포화학 (ko)
- Immunocytochemia (pl)
- Imunocitoquímica (pt)
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| - Immunocytochemistry (ICC) is a common laboratory technique that is used to anatomically visualize the localization of a specific protein or antigen in cells by use of a specific primary antibody that binds to it. The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated fluorophore. ICC allows researchers to evaluate whether or not cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, ICC also allows researchers to determine which sub-cellular compartments are expressing the antigen. (en)
- L’immunocytochimie est une méthode d’analyse des cellules in situ par une technique d’immunofluorescence.Une technique immunochimique dont l'échantillon est une préparation cytologique. (fr)
- 면역세포화학(免疫細胞化學, immunocytochemistry)은 세포에 있는 펩타이드나 단백질 항원의 특정 항원 결정기에 붙는 항체를 이용한 실험 기법이다. 항원 결정기에 달라붙은 항체들은 이차적으로 여러 방법을 통해 인지할 수 있다. 이 방법으로 특정 항원이 대조군과 비교하여 실험군에 존재하는 지 여부를 알 수 있다. 더 나아가서 세포 내 어떤 특정 부분에 그 항원이 존재하는 지도 확인 할 수 있다. (ko)
- Immunocytochemia – metoda wykrywania i lokalizacji składników komórek i tkanek na zasadzie antygen–przeciwciało.Przeciwciało jest białkiem syntetyzowanym przez zwierzęta i ludzi w odpowiedzi na obecność obcej substancji, zwanej antygenem. Białka te mają zdolność swoistego rozpoznania antygenu i wiązania się z jego determinantami antygenowymi (grupami rozpoznawanymi przez przeciwciało) poprzez antydeterminantę zlokalizowaną w obrębie fragmentu Fab. Przeciwciała (zwane również immunoglobulinami) mają swoiste powinowactwo do antygenów, które wywołują ich syntezę. Efektywnymi antygenami są białka, polisacharydy i kwasy nukleinowe. (pl)
- Imunocitoquímica é o conjunto de técnicas que usam anticorpos para identificar células, que funcionam como antigénios, nos tecidos. Esta identificação ocorre devido a reações específicas, interação anticorpo-antigénio, que confere cor aos compostos que se pretendem estudar, permitindo a sua visualização ao microscópio óptico composto. (pt)
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| - Immunocytochemistry (ICC) is a common laboratory technique that is used to anatomically visualize the localization of a specific protein or antigen in cells by use of a specific primary antibody that binds to it. The primary antibody allows visualization of the protein under a fluorescence microscope when it is bound by a secondary antibody that has a conjugated fluorophore. ICC allows researchers to evaluate whether or not cells in a particular sample express the antigen in question. In cases where an immunopositive signal is found, ICC also allows researchers to determine which sub-cellular compartments are expressing the antigen. (en)
- L’immunocytochimie est une méthode d’analyse des cellules in situ par une technique d’immunofluorescence.Une technique immunochimique dont l'échantillon est une préparation cytologique. (fr)
- 면역세포화학(免疫細胞化學, immunocytochemistry)은 세포에 있는 펩타이드나 단백질 항원의 특정 항원 결정기에 붙는 항체를 이용한 실험 기법이다. 항원 결정기에 달라붙은 항체들은 이차적으로 여러 방법을 통해 인지할 수 있다. 이 방법으로 특정 항원이 대조군과 비교하여 실험군에 존재하는 지 여부를 알 수 있다. 더 나아가서 세포 내 어떤 특정 부분에 그 항원이 존재하는 지도 확인 할 수 있다. (ko)
- Immunocytochemia – metoda wykrywania i lokalizacji składników komórek i tkanek na zasadzie antygen–przeciwciało.Przeciwciało jest białkiem syntetyzowanym przez zwierzęta i ludzi w odpowiedzi na obecność obcej substancji, zwanej antygenem. Białka te mają zdolność swoistego rozpoznania antygenu i wiązania się z jego determinantami antygenowymi (grupami rozpoznawanymi przez przeciwciało) poprzez antydeterminantę zlokalizowaną w obrębie fragmentu Fab. Przeciwciała (zwane również immunoglobulinami) mają swoiste powinowactwo do antygenów, które wywołują ich syntezę. Efektywnymi antygenami są białka, polisacharydy i kwasy nukleinowe. (pl)
- Imunocitoquímica é o conjunto de técnicas que usam anticorpos para identificar células, que funcionam como antigénios, nos tecidos. Esta identificação ocorre devido a reações específicas, interação anticorpo-antigénio, que confere cor aos compostos que se pretendem estudar, permitindo a sua visualização ao microscópio óptico composto. (pt)
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