Bartolomé Camacho Zambrano (1510, Villafranca de los Barros, Extremadura, Castile - after 1583, Tunja, New Kingdom of Granada) was a Spanish conquistador who took part in the expedition of the Spanish conquest of the Muisca led by Gonzalo Jiménez de Quesada. He accompanied Gonzalo Suárez Rendón in the foundation of Tunja on August 6, 1539 and settled in the city. In 1583, Bartolomé Camacho Zambrano was together with Francisco de Avendaño.