About: Affinity electrophoresis

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Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. Both qualitative and quantitative information may be obtained through affinity electrophoresis. The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis. The methods are based on changes in the electrophoretic pattern of molecules (mainly macromolecules) through biospecific interaction or complex formation. The interaction or binding of a molecule, charged or uncharged, will normally change the electrophoretic properties of a molecule. Membrane proteins may be identified by a shift in mobility induced by a charged detergent. Nucleic acids or nucleic acid fragments may be characterized by their affin

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rdf:type	yago:WikicatLaboratoryTechniques yago:Ability105616246 yago:Abstraction100002137 yago:Appraisal105733583 yago:Assay105738625 yago:BasicCognitiveProcess105701944 yago:Classification105732756 yago:Cognition100023271 yago:Know-how105616786 yago:Method105660268 yago:Process105701363 yago:PsychologicalFeature100023100 yago:Technique105665146 yago:WikicatProteinMethods yago:WikicatProtein–proteinInteractionAssays
rdfs:label	Affinitätselektrophorese (de) Affinity electrophoresis (en)
rdfs:comment	Die Affinitätselektrophorese umfasst in der Biochemie alle elektrophoretischen Verfahren, bei denen ein verändertes Laufverhalten von Molekülen (z. B. von Proteinkomplexen oder DNA-Protein-Komplexen) durch die Affinität zweier oder mehrerer Moleküle zueinander erzeugt wird. Aufgrund der veränderten Laufgeschwindigkeiten werden affinitätselektrophoretische Verfahren auch als electrophoretic shift assays bezeichnet. (de) Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. Both qualitative and quantitative information may be obtained through affinity electrophoresis. The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis. The methods are based on changes in the electrophoretic pattern of molecules (mainly macromolecules) through biospecific interaction or complex formation. The interaction or binding of a molecule, charged or uncharged, will normally change the electrophoretic properties of a molecule. Membrane proteins may be identified by a shift in mobility induced by a charged detergent. Nucleic acids or nucleic acid fragments may be characterized by their affin (en)
foaf:depiction
dcterms:subject	Molecular biology Laboratory techniques Protein methods Electrophoresis Protein–protein interaction assays
Wikipage page ID	21620243 (xsd:integer)
Wikipage revision ID	1077629498 (xsd:integer)
Link from a Wikipage to another Wikipage	Electromigration Electrophoresis Electrophoretic mobility shift assay Biochemistry Detergent Nucleic acid Ligand Ligands Macromolecules Lectin Molecular biology Capillary electrophoresis Glycan Laboratory techniques Protein methods Affinity chromatography Electrophoresis Protein–protein interaction assays Binding constant Biotechnology Immunoelectrophoresis Complex formation
Link from a Wikipage to an external page	http://onlinelibrary.wiley.com/doi/10.1111/sji.1975.4.issue-s2/issuetoc
sameAs	Affinity electrophoresis Affinity electrophoresis Affinity electrophoresis Affinity electrophoresis Affinity electrophoresis
dbp:wikiPageUsesTemplate	dbt:Reflist dbt:Electrophoresis
thumbnail	wiki-commons:Special:FilePath/Affinityelectrophoreses1.jpg?width=300
has abstract	Affinity electrophoresis is a general name for many analytical methods used in biochemistry and biotechnology. Both qualitative and quantitative information may be obtained through affinity electrophoresis. The methods include the so-called electrophoretic mobility shift assay, charge shift electrophoresis and affinity capillary electrophoresis. The methods are based on changes in the electrophoretic pattern of molecules (mainly macromolecules) through biospecific interaction or complex formation. The interaction or binding of a molecule, charged or uncharged, will normally change the electrophoretic properties of a molecule. Membrane proteins may be identified by a shift in mobility induced by a charged detergent. Nucleic acids or nucleic acid fragments may be characterized by their affinity to other molecules. The methods have been used for estimation of binding constants, as for instance in lectin affinity electrophoresis or characterization of molecules with specific features like glycan content or ligand binding. For enzymes and other ligand-binding proteins, one-dimensional electrophoresis similar to counter electrophoresis or to "rocket immunoelectrophoresis", affinity electrophoresis may be used as an alternative quantification of the protein. Some of the methods are similar to affinity chromatography by use of immobilized ligands. (en) Die Affinitätselektrophorese umfasst in der Biochemie alle elektrophoretischen Verfahren, bei denen ein verändertes Laufverhalten von Molekülen (z. B. von Proteinkomplexen oder DNA-Protein-Komplexen) durch die Affinität zweier oder mehrerer Moleküle zueinander erzeugt wird. Aufgrund der veränderten Laufgeschwindigkeiten werden affinitätselektrophoretische Verfahren auch als electrophoretic shift assays bezeichnet. (de)
gold:hypernym	Name
prov:wasDerivedFrom	wikipedia-en:Affinity_electrophoresis?oldid=1077629498&ns=0
page length (characters) of wiki page	13114 (xsd:nonNegativeInteger)
foaf:isPrimaryTopicOf	wikipedia-en:Affinity_electrophoresis
is Link from a Wikipage to another Wikipage of	Electrophoresis Electrophoresis (disambiguation) Electrophoretic mobility shift assay Methods to investigate protein–protein interactions Protein isoform Chemical affinity Gel electrophoresis Gel electrophoresis of proteins Lectin Capillary electrophoresis Discontinuous electrophoresis Glycan Glycome Glycomics Glycoprotein History of electrophoresis Affinity Immunoelectrophoresis
is Wikipage disambiguates of	Electrophoresis (disambiguation) Affinity
is foaf:primaryTopic of	wikipedia-en:Affinity_electrophoresis

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