The 2016 NCAA Division I Women's Lacrosse Championship was the 35th annual single-elimination tournament to determine the national champion of Division I NCAA women's college lacrosse. The semifinal and championship rounds were played at Talen Energy Stadium (the home of Major League Soccer's Philadelphia Union) in Chester, Pennsylvania from May 27–29, 2016. All other rounds were played at campus sites, usually at the home field of the higher-seeded team, from May 13–22.