The 2001 Cheltenham and Gloucester Trophy was an English limited overs county cricket tournament which was held between 1 May and 1 September 2001. It was the first Cheltenham & Gloucester Trophy following its change of name from the NatWest Trophy, with new sponsors Cheltenham & Gloucester plc. The tournament was won by Somerset who defeated Leicesterhire by 41 runs in the final at Lord's.