U.S. Route 129 (US 129) is an auxiliary route of US 29, which it intersects in Athens, Georgia. US 129 currently runs for 582 miles (937 km) from an intersection with US 19/US 27 ALT/US 98 in Chiefland, Florida, to an interchange with Interstate 40 (I-40) in Knoxville, Tennessee. It passes through the states of Florida, Georgia, North Carolina, and Tennessee. It goes through the cities of Macon, Athens, Gainesville, and Knoxville.