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About: Polymerase cycling assembly     Goto   Sponge   NotDistinct   Permalink

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Type: softwareyago:WikicatAmplifiersyago:Amplifier102705944yago:Artifact100021939yago:ElectronicEquipment103278248yago:Equipment103294048yago:Instrumentality103575240yago:Object100002684yago:PhysicalEntity100001930yago:Whole100003553 New Facet based on Instances of this Class

Polymerase cycling assembly (or PCA, also known as Assembly PCR) is a method for the assembly of large DNA oligonucleotides from shorter fragments. The process uses the same technology as PCR, but takes advantage of DNA hybridization and annealing as well as DNA polymerase to amplify a complete sequence of DNA in a precise order based on the single stranded oligonucleotides used in the process. It thus allows for the production of synthetic genes and even entire synthetic genomes.