Pennsylvania Route 741 (PA 741) is a 26.3-mile-long (42.3 km) state highway that runs through western and southern Lancaster County in the U.S. state of Pennsylvania. The western terminus is along Rohrerstown Road north of an intersection with Commercial Avenue near East Petersburg. The eastern terminus is at PA 41 in Gap. PA 741 heads south from East Petersburg and runs through the western suburbs of Lancaster. The route turns southeast and passes through Millersville before it turns east at New Danville. PA 741 forms a concurrency with U.S. Route 222 (US 222) between Willow Street and Lampeter before it continues east through farmland in the Pennsylvania Dutch Country that is home to several Amish families, passing through Strasburg before reaching Gap.