The Mildura railway line is a heavy rail line in northwestern Victoria, Australia. The line runs from Yelta station to Ballarat station via the settlements of Mildura, Ouyen and Maryborough in an approximate south-southeasterly direction. Initial sections of the line opened from Ballarat in 1874 and the line reached Mildura in 1903. The line is primarily utilised by freight services. V/Line passenger services also operate on the line between Maryborough and Ballarat.