Dayr Ayyub (Arabic: دير أيوب) was a Palestinian Arab village in the Ramle Subdistrict. It was depopulated during the 1947–48 Civil War in Mandatory Palestine on March 6, 1948 by the Givati and Sheva' brigades of Operation Nachshon. It was located 17.5 km southeast of Ramla, situated close to Bab al-Wad. On attack, the village was defended by the Jordanian Army but was mostly destroyed with the exception of a few houses and the village cemetery.