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Statements

Subject Item
dbr:Ribonucleoprotein_Networks_Analyzed_by_Mutational_Profiling
rdfs:label
Ribonucleoprotein Networks Analyzed by Mutational Profiling
rdfs:comment
Ribonucleoprotein Networks Analyzed by Mutational Profiling (RNP-MaP) is a strategy for probing RNA-protein networks and protein binding sites at a nucleotide resolution. Information about RNP assembly and function can facilitate a better understanding of biological mechanisms. RNP-MaP uses NHS-diazirine (SDA), a hetero-bifunctional crosslinker, to freeze RNA-bound proteins in place. Once the RNA-protein crosslinks are formed, MaP reverse transcription is then conducted to reversely transcribe the protein-bound RNAs as well as introduce mutations at the site of RNA-protein crosslinks. Sequencing results of the cDNAs reveal information about both protein-RNA interaction networks and protein binding sites.
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dbr:Ribose dbr:Correlation dbc:Genetics_techniques dbr:Peptide_bond dbc:RNA dbr:Ribonucleoprotein dbr:N-Hydroxysuccinimide dbr:RNA dbr:Reverse_transcription dbr:Mass_spectrometry n11:RNP-MaP_workflow.png dbr:PAR-CLIP dbr:Ultraviolet dbr:Cross-linking_immunoprecipitation dbr:Sequencing dbr:Lysis dbr:Nucleotide dbr:Cross-link dbr:Normalization_(statistics) dbr:HITS-CLIP dbr:Amide dbr:Read_depth dbr:Protein dbr:High_throughput_sequencing dbr:Lysine dbr:Mutation dbr:ICLIP dbr:RNA-binding_protein dbr:Quantitative_proteomics dbr:Arginine dbr:Diazirine dbr:Immunoprecipitation dbr:Ester dbr:Amine dbr:Complementary_DNA dbr:Sequencing_coverage
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Ribonucleoprotein Networks Analyzed by Mutational Profiling (RNP-MaP) is a strategy for probing RNA-protein networks and protein binding sites at a nucleotide resolution. Information about RNP assembly and function can facilitate a better understanding of biological mechanisms. RNP-MaP uses NHS-diazirine (SDA), a hetero-bifunctional crosslinker, to freeze RNA-bound proteins in place. Once the RNA-protein crosslinks are formed, MaP reverse transcription is then conducted to reversely transcribe the protein-bound RNAs as well as introduce mutations at the site of RNA-protein crosslinks. Sequencing results of the cDNAs reveal information about both protein-RNA interaction networks and protein binding sites.
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