John MacFarlane (31 July 1813 – 6 July 1873) was a Scottish-born Australian physician and politician. He was born in Glasgow and studied medicine at Glasgow University, qualifying in 1837. He worked as a ship's doctor until 1840, when he settled in Sydney and opened a practice. In 1847 he married Agnes Grace Johnstone. He was honorary physician at Sydney Infirmary from 1847 to 1869 and an examiner for the University of Sydney in 1856; he was also awarded an honorary degree from the University of Melbourne. In 1860 he was president of the Australian Medical Association. He was a member of the New South Wales Legislative Council from 1858 to 1861 and from 1861 to 1870.
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