The Men's 400 metres T54 event at the 2016 Summer Paralympics took place at the Estádio Olímpico João Havelange on 11 and 12 September. It featured 20 athletes. The T54 category is for wheelchair athletes with no leg function, but near full arm function and reasonable to normal trunk function. Typically this may be caused by a lower spinal cord injury or spinal cord birth defect.