The MTT assay is a colorimetric assay for assessing cell viability. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple color.

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dbpedia-owl:abstract
  • The MTT assay is a colorimetric assay for assessing cell viability. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple color. Other closely related tetrazolium dyes including XTT, MTS and the WSTs, are used in conjunction with the intermediate electron acceptor, 1-methoxy PMS. With WST-1, which is cell-impermeable, reduction occurs outside the cell via plasma membrane electron transport. Tetrazolium dye assays can also be used to measure cytotoxicity (loss of viable cells) or cytostatic activity (shift from proliferative to resting status) of potential medicinal agents and toxic materials. MTT assays are usually done in the dark since the MTT reagent is sensitive to light.
  • Der MTT-Test ist ein Test zur Bestimmung der Zellviabilität.
  • Le test MTT est une méthode rapide de numération des cellules vivantes.
  • Il saggio MTT, dove l'acronimo indica il composto bromuro di 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolio, è un saggio colorimetrico standard per la misurazione dell'attività degli enzimi che riducono l'MTT a formazano, conferendo alla sostanza un colore blu/violaceo. Ciò accade prevalentemente nei mitocondri; può essere utilizzato per determinare la citotossicità di farmaci o altri tipi di sostanze chimicamente attive e potenzialmente tossiche.L'enzima mitocondriale succinato deidrogenasi, è attivo infatti soltanto nelle cellule vive, e la sua funzione consiste nel tagliare l'anello di tetrazolio dell'MTT (sostanza di colore giallo) con la formazione, di conseguenza, di formazano (un sale blu).Tale reazione è valutata e misurata mediante la lettura spettrofotometrica del campione, alla lunghezza d'onda di 570 nm.
  • MTT試験(エムティーティーしけん、MTTアッセイ、英: MTT assay)およびMTS試験は、MTTや類似の色素(XTT, MTS, WST)をホルマザン色素(紫色)へ還元する酵素活性を測定する比色定量法である。この方法により培養細胞の生存率や増殖率を試験することが可能である。様々な試薬(医薬品候補など)や毒物の細胞毒性を評価することにも用いられる。
  • 細胞存活率分析 (英语:MTT assay,全名:(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide),是一種用於測量細胞的活性之試驗。
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  • Der MTT-Test ist ein Test zur Bestimmung der Zellviabilität.
  • Le test MTT est une méthode rapide de numération des cellules vivantes.
  • MTT試験(エムティーティーしけん、MTTアッセイ、英: MTT assay)およびMTS試験は、MTTや類似の色素(XTT, MTS, WST)をホルマザン色素(紫色)へ還元する酵素活性を測定する比色定量法である。この方法により培養細胞の生存率や増殖率を試験することが可能である。様々な試薬(医薬品候補など)や毒物の細胞毒性を評価することにも用いられる。
  • 細胞存活率分析 (英语:MTT assay,全名:(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide),是一種用於測量細胞的活性之試驗。
  • The MTT assay is a colorimetric assay for assessing cell viability. NAD(P)H-dependent cellular oxidoreductase enzymes may, under defined conditions, reflect the number of viable cells present. These enzymes are capable of reducing the tetrazolium dye MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide to its insoluble formazan, which has a purple color.
  • Il saggio MTT, dove l'acronimo indica il composto bromuro di 3-(4,5-dimetiltiazol-2-il)-2,5-difeniltetrazolio, è un saggio colorimetrico standard per la misurazione dell'attività degli enzimi che riducono l'MTT a formazano, conferendo alla sostanza un colore blu/violaceo.
rdfs:label
  • MTT assay
  • MTT-Test
  • Test MTT
  • Saggio MTT
  • MTT試験
  • MTT試驗
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