An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity (the analyte). The analyte can be a drug or biochemical substance or a cell in an organism or organic sample. The measured entity is generally called the analyte, or the measurand or the target of the assay. The assay usually aims to measure an intensive property of the analyte and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of some effect in comparison to a standard, etc.).

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  • An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity (the analyte). The analyte can be a drug or biochemical substance or a cell in an organism or organic sample. The measured entity is generally called the analyte, or the measurand or the target of the assay. The assay usually aims to measure an intensive property of the analyte and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of some effect in comparison to a standard, etc.). If the assay involves addition of exogenous reactants (the reagents) then their quantities are kept fixed (or in excess) so that the quantity (and quality) of the target is the only limiting factor for the reaction/assay process, and the difference in the assay outcome is used to deduce the unknown quality or quantity of the target in question. Some assays (e.g., biochemical assays) may be similar to or have overlap with chemical analysis and titration. But generally, assays involve biological material or phenomena which tend to be intrinsically more complex either in composition or in behavior or both. Thus reading of an assay may be quite noisy and may involve greater difficulties in interpretation than an accurate chemical titration. On the other hand, older generation qualitative assays, especially bioassays, may be much more gross and less quantitative (e.g., counting death or dysfunction of an organism or cells in a population, or some descriptive change in some body part of a group of animals). Assays have become a routine part of modern medical, environmental, pharmaceutical, forensic and many other businesses at various scales from industrial to curbside or field level. Those assays that are very highly commercially demanded have been well investigated in research and development sectors of professional industries, undergone generations of development and sophistication, and become copyrighted intellectual properties via highly competitive process patenting. Such industrial scale assays as these are often done in well equipped laboratories and with automated organization of the procedure—from ordering an assay to pre-analytic sample processing (sample collection, necessary manipulations e.g. spinning for separation or other processes, aliquoting if necessary, storage, retrieval, pipetting/aspiration etc.). Analytes are generally tested in high throughput AutoAnalyzers, and the results are verified and automatically returned to ordering service providers and end users. These are made possible through use of advanced Laboratory informatics system that interfaces with multiple computer terminals with end users, central servers, the physical autoanalyser instruments, and other automata. (en)
  • Das Wort Assay (deutsch: Test, Probe) stammt aus dem englischen Sprachraum und bedeutet dort allgemein eine Untersuchung zum Nachweis bestimmter Substanzen (z. B. Metalle). In der deutschen Sprache bezeichnet Assay vor allem in der Labormedizin und der pharmazeutischen Forschung einen standardisierten Reaktionsablauf zum Nachweis einer Substanz mit einer spezifischen Methode. Insbesondere sind damit molekularbiologische Methoden gemeint. Es gibt keine Besonderheit, die einen Assay gegenüber anderen Nachweisverfahren auszeichnet, allenfalls dass das Vorkommen der Bezeichnung die Herkunft des Namens oder der Methode aus dem englischen Sprachraum anzeigt. In vielen Fällen wurde Assay durch andere Wörter ersetzt (z. B. Bradford-Test). (de)
  • Un ensayo químico o análisis químico es un procedimiento para medir la concentración o cualquier otra propiedad química de una sustancia o material. Sinónimos: prueba, análisis, test, examen. Hay numerosos tipos de ensayos. Un ensayo no destructivo es aquel que mantiene la integridad de la muestra. En otros donde se produce una reacción química, el test se realiza en una fracción representativa de la muestra llamada alícuota. (es)
  • Ensaio é um processo investigativo (analítico) laboratorial que é utilizado pela, química, medicina, farmacologia, biologia ambiental, e biologia molecular para avaliar qualitativamente ou quantitativamente a presença, a quantidade ou a atividade funcional de um analito. O analito pode ser uma droga, substância bioquímica, célula de um organismo ou uma amostra biológica. (pt)
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  • Un ensayo químico o análisis químico es un procedimiento para medir la concentración o cualquier otra propiedad química de una sustancia o material. Sinónimos: prueba, análisis, test, examen. Hay numerosos tipos de ensayos. Un ensayo no destructivo es aquel que mantiene la integridad de la muestra. En otros donde se produce una reacción química, el test se realiza en una fracción representativa de la muestra llamada alícuota. (es)
  • Ensaio é um processo investigativo (analítico) laboratorial que é utilizado pela, química, medicina, farmacologia, biologia ambiental, e biologia molecular para avaliar qualitativamente ou quantitativamente a presença, a quantidade ou a atividade funcional de um analito. O analito pode ser uma droga, substância bioquímica, célula de um organismo ou uma amostra biológica. (pt)
  • An assay is an investigative (analytic) procedure in laboratory medicine, pharmacology, environmental biology and molecular biology for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity (the analyte). The analyte can be a drug or biochemical substance or a cell in an organism or organic sample. The measured entity is generally called the analyte, or the measurand or the target of the assay. The assay usually aims to measure an intensive property of the analyte and express it in the relevant measurement unit (e.g. molarity, density, functional activity in enzyme international units, degree of some effect in comparison to a standard, etc.). (en)
  • Das Wort Assay (deutsch: Test, Probe) stammt aus dem englischen Sprachraum und bedeutet dort allgemein eine Untersuchung zum Nachweis bestimmter Substanzen (z. B. Metalle). In der deutschen Sprache bezeichnet Assay vor allem in der Labormedizin und der pharmazeutischen Forschung einen standardisierten Reaktionsablauf zum Nachweis einer Substanz mit einer spezifischen Methode. Insbesondere sind damit molekularbiologische Methoden gemeint. (de)
rdfs:label
  • Assay (en)
  • Assay (de)
  • Ensayo químico (es)
  • Ensaio (ciência) (pt)
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