Maria Maddalena of Austria (Maria Magdalena; 7 October 1589 – 1 November 1631) was Grand Duchess of Tuscany from the accession of her husband, Cosimo II, in 1609 until his death in 1621. With him, she had eight children, including a duchess of Parma, a grand duke of Tuscany, and an archduchess of Further Austria. Born in Graz, she was the youngest daughter of Charles II, Archduke of Inner Austria, and his wife Maria Anna of Bavaria. During the minority of her son, Grand Duke Ferdinando, she and her mother-in-law acted as regents from 1621 to 1628. She died on 1 November 1631 in Passau.