About: Third-generation sequencing

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Third-generation sequencing (also known as long-read sequencing) is a class of DNA sequencing methods currently under active development. Third generation sequencing technologies have the capability to produce substantially longer reads than second generation sequencing, also known as next-generation sequencing. Such an advantage has critical implications for both genome science and the study of biology in general. However, third generation sequencing data have much higher error rates than previous technologies, which can complicate downstream genome assembly and analysis of the resulting data. These technologies are undergoing active development and it is expected that there will be improvements to the high error rates. For applications that are more tolerant to error rates, such as struc

Attributes	Values
rdfs:label	Third-generation sequencing (en)
rdfs:comment	Third-generation sequencing (also known as long-read sequencing) is a class of DNA sequencing methods currently under active development. Third generation sequencing technologies have the capability to produce substantially longer reads than second generation sequencing, also known as next-generation sequencing. Such an advantage has critical implications for both genome science and the study of biology in general. However, third generation sequencing data have much higher error rates than previous technologies, which can complicate downstream genome assembly and analysis of the resulting data. These technologies are undergoing active development and it is expected that there will be improvements to the high error rates. For applications that are more tolerant to error rates, such as struc (en)
foaf:depiction
dcterms:subject	Biotechnology DNA sequencing methods Molecular biology Molecular biology techniques
Wikipage page ID	53363521 (xsd:integer)
Wikipage revision ID	1123060729 (xsd:integer)
Link from a Wikipage to another Wikipage	Caenorhabditis elegans Epigenetics Metagenomics De Bruijn graph Antimicrobial resistance DNA DNA methylation Biotechnology Fungus Embryonic stem cell Sequence assembly Paired-end tag Pathogen Massive parallel sequencing Autism Bacteria Helicos Biosciences 5-Methylcytosine 454 Life Sciences DNA sequencing methods Molecular biology Molecular biology techniques DNA sequencing Alternative splicing Escherichia coli Eukaryote Oxford Nanopore Technologies Pacific Biosciences Central dogma of molecular biology Hybrid genome assembly ChIP-sequencing Ebola virus Hidden Markov model Zero-mode waveguide Illumina (company) Mann–Whitney U test Phylogenetics Methyl group Methyltransferase dbr:Helicos_single_molecule_fluorescent_sequencing Nanopore sequencing Sanger sequencing Parasitism Ribosomal RNA Transcriptome Methylome MinION SMRT sequencing First-generation sequencing Transcriptomics Second-generation sequencing
Link from a Wikipage to an external page	https://cole-trapnell-lab.github.io/cufflinks/ https://www.pacb.com/smrt-science/smrt-sequencing/smrt-sequencing-modes/ https://nanoporetech.com/products/minion
sameAs	Third-generation sequencing Third-generation sequencing Third-generation sequencing
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thumbnail	wiki-commons:Special:FilePath/MinION_Portable_Gene_Sequencer_IMG_20200219_170422318.jpg?width=300
date	January 2020 (en)
section	yes (en)
has abstract	Third-generation sequencing (also known as long-read sequencing) is a class of DNA sequencing methods currently under active development. Third generation sequencing technologies have the capability to produce substantially longer reads than second generation sequencing, also known as next-generation sequencing. Such an advantage has critical implications for both genome science and the study of biology in general. However, third generation sequencing data have much higher error rates than previous technologies, which can complicate downstream genome assembly and analysis of the resulting data. These technologies are undergoing active development and it is expected that there will be improvements to the high error rates. For applications that are more tolerant to error rates, such as structural variant calling, third generation sequencing has been found to outperform existing methods, even at a low depth of sequencing coverage. (en)
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foaf:isPrimaryTopicOf	wikipedia-en:Third-generation_sequencing
is Link from a Wikipage to another Wikipage of	Prunus × nudiflora Metatranscriptomics David W. Deamer 16S ribosomal RNA GATC Biotech Long-read sequencing Clinical metagenomic sequencing Sequence assembly Massive parallel sequencing DNA sequencing Zaire ebolavirus List of RNA-Seq bioinformatics tools Read (biology) Helmy Eltoukhy Bacterial genome Hybrid genome assembly

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