In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. The major advantage of explant culture is the maintenance of near in vivo environment in the laboratory for a short duration of time. This experimental setup allows investigators to perform experiments and easily visualize the impact of tests.
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| - Explanto (es)
- Explant culture (en)
- Explant (fr)
- Espianto (it)
- Explante (pt)
- Эксплант (ru)
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| - El explanto o explante es un tejido vivo separado de su órgano propio y transferido a un medio artificial de crecimiento. En el caso particular de la biotecnología vegetal, el explanto es un pequeño fragmento de una planta que se escinde y se prepara de forma aséptica para su cultivo en un medio nutritivo y que, por ende, funciona como generadora de nuevas plantas a través de cultivo de tejidos in vitro. (es)
- Dans le domaine du clonage et plus généralement des biotechnologies et de la production végétale, un « explant » est le matériel végétal de base qui servira à produire des clones végétaux à partir d'une « plante-mère » ou éventuellement secondairement des greffons. Étymologiquement, le mot explant évoque un sens contraire à celui d'implant. (fr)
- Um explante é uma célula, tecido ou órgão de uma planta usado para iniciar culturas in vitro. Os explantes devem ser retirados de partes de plantas em crescimento activo que não tenham passado por qualquer tipo de "stress" como seca, temperaturas demasiadamente altas ou baixas, carência mineral e ataque de pragas ou doenças. (pt)
- Эксплант (лат. explanto — вырываю что-нибудь, растущее с корнем) — группа клеток, отделенная от материнского организма. Используется в биологических исследованиях, связанных с микроклональным размножением растений. У двудольных растений, эксплантами могут являться, например, части гипокотилей, стеблей, корней, семядоли, пыльники и прочее. При культивировании эксплантов на питательных средах различного состава, можно наблюдать или органогенез (образование корней, побегов и пр.) (ru)
- In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. The major advantage of explant culture is the maintenance of near in vivo environment in the laboratory for a short duration of time. This experimental setup allows investigators to perform experiments and easily visualize the impact of tests. (en)
- Un espianto è un tessuto vivo separato dal proprio organo e trasferito in un mezzo artificiale di crescita. Può essere una porzione del germoglio, delle foglie o di alcune cellule di una pianta, oppure può essere una qualsiasi parte del tessuto di un animale. Tale procedura trova vasta applicazione nel campo delle biotecnologie (soprattutto vegetali) e prende il nome di coltura artificiale dei tessuti. (it)
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| - In biology, explant culture is a technique to organotypically culture cells from a piece or pieces of tissue or organ removed from a plant or animal. The term explant can be applied to samples obtained from any part of the organism. The extraction process is extensively sterilized, and the culture can be typically used for two to three weeks. The major advantage of explant culture is the maintenance of near in vivo environment in the laboratory for a short duration of time. This experimental setup allows investigators to perform experiments and easily visualize the impact of tests. This ex vivo model requires a highly maintained environment in order to recreate original cellular conditions. The composition of extracellular matrix, for example, must be precisely similar to that of in vivo conditions in order to induce naturally observed behaviors of cells. The growth medium also must be considered, as different solutions may be needed for different experiments. The tissue must be placed and harvested in an aseptic environment such as sterile laminar flow tissue culture hood. The samples are often minced, and the pieces are placed in a cell culture dish containing growth media. Over time, progenitor cells migrate out of the tissue onto the surface of the dish. These primary cells can then be further expanded and transferred into fresh dishes through micropropagation. Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture, or blastocyst implant culture. (en)
- El explanto o explante es un tejido vivo separado de su órgano propio y transferido a un medio artificial de crecimiento. En el caso particular de la biotecnología vegetal, el explanto es un pequeño fragmento de una planta que se escinde y se prepara de forma aséptica para su cultivo en un medio nutritivo y que, por ende, funciona como generadora de nuevas plantas a través de cultivo de tejidos in vitro. (es)
- Dans le domaine du clonage et plus généralement des biotechnologies et de la production végétale, un « explant » est le matériel végétal de base qui servira à produire des clones végétaux à partir d'une « plante-mère » ou éventuellement secondairement des greffons. Étymologiquement, le mot explant évoque un sens contraire à celui d'implant. (fr)
- Un espianto è un tessuto vivo separato dal proprio organo e trasferito in un mezzo artificiale di crescita. Può essere una porzione del germoglio, delle foglie o di alcune cellule di una pianta, oppure può essere una qualsiasi parte del tessuto di un animale. In breve il tessuto è raccolto in maniera asettica, spesso triturato, e i frammenti sono posti in un recipiente di coltura cellulare contenente un mezzo nutritivo. Col tempo, le cellule progenitrici migrano fuori dal tessuto sulla superficie del recipiente. Queste cellule primarie possono poi essere ulteriormente espanse e trasferite in nuovi recipienti. Tale procedura trova vasta applicazione nel campo delle biotecnologie (soprattutto vegetali) e prende il nome di coltura artificiale dei tessuti. Un'altra forma di coltura degli espianti è la coltivazione delle stesse parti di tessuto, in cui le cellule sono lasciate nella loro matrice extracellulare circostante per riprodurre in maniera più accurata l'ambiente in vivo, ad es. la coltura di un espianto di cartilagine. (it)
- Um explante é uma célula, tecido ou órgão de uma planta usado para iniciar culturas in vitro. Os explantes devem ser retirados de partes de plantas em crescimento activo que não tenham passado por qualquer tipo de "stress" como seca, temperaturas demasiadamente altas ou baixas, carência mineral e ataque de pragas ou doenças. (pt)
- Эксплант (лат. explanto — вырываю что-нибудь, растущее с корнем) — группа клеток, отделенная от материнского организма. Используется в биологических исследованиях, связанных с микроклональным размножением растений. У двудольных растений, эксплантами могут являться, например, части гипокотилей, стеблей, корней, семядоли, пыльники и прочее. При культивировании эксплантов на питательных средах различного состава, можно наблюдать или органогенез (образование корней, побегов и пр.) (ru)
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