The Women's 400 metres Hurdles at the 1988 Summer Olympics in Seoul, South Korea had an entry list of 35 competitors, with five qualifying heats and two semifinals (16) before the final (8) took place on Wednesday September 28, 1988. In Seoul, Fiedler set a new Olympic record in the heats, running 54.58 secs. Flintoff-King then further improved the Olympic record to 54.00 in the first semifinal, finishing just ahead of the little-known Soviet Tatyana Ledovskaya, who ran 54.01. Fiedler won the second semifinal in 54.28, with world champion Busch fourth.